Restriction digests of the clone give the following sizes (kb): HindIII–5.6, PstI–4.8, 0.45, 0.35; EcoRI–5.6. (ATCC staff) Shuttle expression vector allowing production of a fusion protein containing an amino terminal c-myc epitope tag (FQKLISEEDLN), that can be used for immunological detection of the recombinant protein in transfected cells. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Matus A.L. Hosts: E.coli, E.coli HB101, mammalian cells.