大鼠前列腺素F(PGF)ELISA試劑盒,規格:48T/96T,大鼠前列腺素F(PGF)ELISA試劑盒原理,大鼠前列腺素F(PGF)ELISA試劑盒說明書,大鼠前列腺素F(PGF)ELISA試劑盒包裝,英文名稱,rat Prostaglandin F,PG-F ELISA Kit
包裝:液體盒裝(48T/96T)
保存條件:2-8℃(長期不使用時,部分試劑應放在-20℃條件下)
大鼠前列腺素F(PGF)ELISA試劑盒存儲運輸:低溫避光(請勿重壓,輕拿輕放)
【原理】
大鼠前列腺素F(PGF)ELISA試劑盒是利用固相夾心法酶聯免疫吸附實驗(ELISA)已知濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測。先將生物素標記的抗體同時溫育。洗滌後,加入親和素標記過的HRP。再經過溫育和洗滌,去除未結合的酶結合物,然後加入底物A、B,和酶結合物同時作用。產生顏色。顏色的深淺和樣品中的濃度呈比例關系。
具體可總結為:
①抗原或抗體能吸附到固相載體表面,並保持其免疫學活性;
②抗原或抗體可通過共價鍵與酶連接形成酶結合物,仍保持其免疫學和酶學活性;
③酶結合物與相應抗原或抗體結合後,可根據加入底物的顏色反應來判定是否有免疫反應的存在,而且顏色反應的深淺是與標本中相應抗原或抗體的量成正比例,因此,可以按底物顯色的程度顯示試驗結果。
ELISA常用的方法主要包括雙抗體夾心法、間接法、競爭法以及BAS-ELISA。
【大鼠前列腺素F(PGF)ELISA試劑盒概念】
酶聯免疫吸附試驗(enzyme-linked immune sorbent assay, ELISA)是Engvall和Van weemen於1971年提出的。該法是以固相載體吸附技術和免疫酶技術相結合建立的一種檢測方法,其操作簡便,無需特殊設備,並具有高度的敏感性和準確性,所以受到大傢的廣泛重視,以致在較短時間內,於國內外均取得瞭較快的進展。
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【大鼠前列腺素F(PGF)ELISA試劑盒特點】
ELISA實驗特點:靈敏度高,檢測能力可達10-6mg/ml水平;大鼠前列腺素F(PGF)ELISA試劑盒應用范圍廣,既能檢測抗原又能檢測抗體,大鼠前列腺素F(PGF)ELISA試劑盒既能定性又能定量;有效期長,酶標抗體於普通冰箱中可保存一年以上,效價不下降,大鼠前列腺素F(PGF)ELISA試劑盒制成的標本,可長久保存,供隨時復查用。
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